In the 1530s  I showed people the importance of surgery and anatomy and wrote a pamphlet on blood letting, a popular treatment for common illnesses. This pamphlet was supported by my  knowledge on the blood system and showed how anatomical dissection could also be used in test speculation and emphasized the significance on understanding the human body in medicine. In 1539 Paduan became interested in my work and donated bodies from executed criminals to my human dissections. Now I was able to make repeated and corresponding dissections on humans and in 1543 i published De Humani Corporis Fabrica based on my knowledge.

My work overthrew many of the hitherto-uncontested doctrines of the second-century anatomist Galen, and caused a storm of criticism from other anatomists because I wanted to prove them wrong with some of there assumptions about the human body. Galen studied the animal body or mainly apes instead of the human to make his discoveries on human anatomy . Some of my influences were Jacques Dubois and Jean Fernel, when I studied under them in university I found my interest in anatomy. Also my family had been established in  medicine for several generations.

In 1543 I contributed to humanish by writing seven volumes on the human body called De humani corporis fabrica (The structures of the human body). All were illustrated by my own drawing’s , this book described several organs and for the first time the thalmus. This was the most accurate and detailed anatomical text ever to be produced.